To detect the effect of reverse in transfected cells by allicin treated. Two different short hairpin RNAs were designed and constructed in a pSilencer 3.1-H1 neo plasmid. The shRNA recombinant plasmids were transfected into HT9 leukemia cells. Flow cytometry was used to select the stably transfected sells. The cell viability of the cells was assessed using MTT assay, flow cytometry was used to monitor cell cycle and apoptosis. Inverted fluorescence microscope and trasmission electron microscope were used to examine the cytomorphology and ultrastructural changes of cells. The results indicated that compared with HT9 cells, HT9-3.1-2 cells, HT9 cells transfected with shRNA recombinant plasmids, had lower cell viability and higher levels of apoptosis after treated with allicin. The characteristic features of apoptosis were more significantly. The results indicate that allicin can induce cell apoptosis of HT9 and HT9-3.1-2 cells, and after stable transfection with the shRNA vectors, the multidrug resistance in HT9 cells was partly reversed.