Paper Title:
Research of Immobilization of Transglutaminase on Polypropylene Microporous Membranes
  Abstract

Activated ozone as the carrier,crylic acid as the monomer and glutaraldehyde as the cross-linking agent were used to research the immobilization of transglutaminase on polypropylene microporous membranes. The effect of ozone activation time,grafting time,grafting temperature,monomer concentration and ammonium ferrous sulfate concentration on grafting degree were studied, the Immobilization conditions were also studied. We found the optimal reaction conditions as following: consistency of hexamethylendiamine was 15%,at 50°C for 120min and consistency,at 30°C for 45minites,consistency of enzyme was 15mg/mL at 4°C. So the immobilized amount of protein could come to 30.23mg/g membrane and the activity of immobilized MTG come to 16.9U/g membrane.

  Info
Periodical
Advanced Materials Research (Volumes 341-342)
Edited by
Liu Guiping
Pages
338-344
DOI
10.4028/www.scientific.net/AMR.341-342.338
Citation
Z. G. Na, X. F. Wang, Y. Q. Ma, L. Qian, H. Y. Yan, "Research of Immobilization of Transglutaminase on Polypropylene Microporous Membranes", Advanced Materials Research, Vols. 341-342, pp. 338-344, 2012
Online since
September 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Jun Fu Wei, Kong Yin Zhao, Huan Zhang, Xiao Lei Wang, Li Chen
Abstract:A weak acid cation exchange fiber was prepared by γ-ray pre-radiation induced grafting of acrylic acid (AA) onto polypropylene (PP) non-woven...
1764
Authors: Chun Xiang Lin, Ming Hua Liu, Huai Yu Zhan
Abstract:The spherical cellulose adsorbent was prepared by grafting acrylic acid onto the spherical cellulose beads prepared by NMMO method. The...
899
Authors: Hua Feng Zhang, Yu Zhong Zhang, Li Gang Lin, Xiao Li Ding, Hong Li
Abstract:Polyvinylidene fluoride (PVDF) hydrophobic membrane has been prepared by phase inversion method. The effects of concentration of PVDF,...
338
Authors: Lin Bo Li, Juan Qin Xue, Tao Hong, Jun Yang, Xing Wang
Abstract:The separation of cobalt from zinc sulfate solutions by oxidation-precipitation process using peroxy-monosulfuric acid as oxidant has been...
145
Authors: Su Rong Hu, Chao Wang, Qi Feng Wei
Chapter 15: Chemical Metallurgy and Mining Explorations
Abstract:An attempt has been made to study the pretreatment of sulfidic refractory gold ore with pyrolusite. The influences of various parameters were...
2891