Paper Title:
Expression and Inhibition Analysis of Recombinant Protease Inhibitor of Solamum Americanum in E. Coli
  Abstract

SaPIN2a, the proteinase inhibitor of nightshade (Solanum americanum), has been proposed to regulate proteolysis in phloem development. In this study, we expressed and characterized recombinant SaPIN2a in Escherichia coli. Purified recombinant SaPIN2a (rSaPIN2a) had a strong inhibitory effect on serine proteinase chymotrypsin (IC50 36.1 nmol/L), but its inhibitory activities toward trypsin (IC50 398.6 nmol/L) and especially toward subtilisin (IC50 5004 nmol/L) were low. It did not inhibit cysteine proteinase papain and aspartic proteinase cathepsin D. rSaPIN2a was a competitive inhibitor of trypsin, and a noncompetitive inhibitor of chymotrypsin and subtilisin.

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 3: Biology-Related Materials Research in Environmental Protection
Edited by
David Wang
Pages
1053-1058
DOI
10.4028/www.scientific.net/AMR.343-344.1053
Citation
Z. Y. Wang, J. Li, M. Z. Li, T. C. Zhang, "Expression and Inhibition Analysis of Recombinant Protease Inhibitor of Solamum Americanum in E. Coli", Advanced Materials Research, Vols. 343-344, pp. 1053-1058, 2012
Online since
September 2011
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Price
$32.00
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