Samples on a regular basis, and the use of SDSPAGE and Western Blotting methods was inspected to express situation in the induction of heat shock protein (both Hsp27 and Hsp40) of the body tissue of earthworms (Eisenia foetide) exposed in the soil by respectively adding Atrazine, Chlorotoluron; Acetochlor, Butachlor. According to the gel imaging system for gray-scale scanner, with the increase of the concentration of herbicides, the relative expression of both Hsps showed the phenomenon of induction in low concentration and suppression in high concentrations within the range of tested concentration. With the extension of the processing time, both Hsps were first induced suppression of the general trend and there was good exposure time – coercing effect relationship. The relative expression of HSP27 and HSP40 exposed by Atrazine, Chlorotoluron, Acetochlor and Butachlor were 2.21, 1.98, 2.06, 2.11 times and 2.32, 1.99, 2.15, 2.24 times of the control group. The sensitive ability of the organization of HSP27 and HSP40 to four herbicides was in the order of Atrazine > Butachlor > Acetochlor > Chlorotoluron. The relative expression of Hsp40 slightly was a little bit larger than Hsp27.