Paper Title:
Screening of Lipid High Producing Mutant from Rhodotorula Glutinis by Low Ion Implantation and Study on Lipid Extration Technique
  Abstract

The paper studied on parameters of ion implantation into lipid producing strain Rhodotorula glutinis and lipid extration technology. It was found that the strain had a higher positive mutation rate when the output power was 10keV and the dose of N+ implantation was 80×2.6×1013 ions/cm2. Then a high-yield mutant strain D30 was obtained and it’s lipid yield which was 3.10 g/L increased by 33.05% than that of the original. Additionally, statistically-based experimental designs were applied for the optimization of lipid extraction by acid-heating coupling ultrasonic technique. By a Plackett-Burman design, it was found that three factors, treatment time of HCl (p=0.036) , ultrasonic time (p=0.0105) and rate of extracting solution (VCHCl3:VCH3OH) (p=0.0105), had significant effect on lipid extraction. Subsequently, these significant factors were optimized using response surface methodology (RSM), and the optimized parameters of lipid extraction were as follows: 34 min for treatment time of HCl, 7.5 min for ultrasonic treating time, 1.9:1 for rate of extracting solution (VCHCl3:VCH3OH). Finally the fermentation characteristic of high-yield mutation strain D30 was studied, when fermentation time was 10 d, it’s lipid yield increased to 7.81 g/L

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 3: Biology-Related Materials Research in Environmental Protection
Edited by
David Wang
Pages
1172-1181
DOI
10.4028/www.scientific.net/AMR.343-344.1172
Citation
S. C. Li, H. X. Liu, S. B. Gu, M. Wang, Z. Y. Zhu, D. D. Wang, "Screening of Lipid High Producing Mutant from Rhodotorula Glutinis by Low Ion Implantation and Study on Lipid Extration Technique", Advanced Materials Research, Vols. 343-344, pp. 1172-1181, 2012
Online since
September 2011
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Price
$32.00
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