To study the effect of different sucrose concentration on bulblet formation, three kinds of Lilium plantlets, Lilium davidii var. unicolor, L. longiflorum ‘White Heaven’ and L. OT ‘Yelloween’ were inoculated on media containing different sugar concentration. It was found that L. davidii var. unicolo had 100% percent in bulblet formation at the sucrose concentration 100g/L, while the diameter and weight increment of bulblets came to the maximum. Both ‘White Heaven’ and ‘Yelloween’ had 100% percent of bulblets formation on the sucrose concentration at 80g/L and 100 g/L. But the diameter and weight increment of bulblets in different varieties had different results. The maximum of ‘White Heaven’ bulblets could be obtained at sucrose concentration 60g/L, while ‘Yelloween’ at 80g/L. It was concluded that sucrose concentrations at 40~80g/L had a positive impact on these 3 lilies bulblets formation in vitro cultures.