In agricultural industry, waste management is a crucial issue in developing countries as agricultural wastes have become one of the main causes of the globally environmental pollution. Thus, how to effectively utilize these wastes will substantially reduce environmental pollution and lower resources losses. Instead of adding chemicals, this research used different species of bananas in shake flask cultures of Agaricus blazei to yield the same amount of polysaccharide as in other common medium. The dry weight of mycelia and polysaccharide had a 0.06 mg/ml change in the Agaricus blazei mycelia by adding banana wastes. In addition, the mycelium dry weight and polysaccharide production were in positive correlation.