Role of nitric oxide(NO) and hydrogen peroxide(H2O2) in eATP-inhibited reduction of in vitro pollen germination and tube growth of Paulownia tomentosa Steud. were studied. Results showed that exposure of the pollen to a series of eATP (0.2, 0.4, 0.6, 0.8, 1.0 mM) concentration for 3h resulted in not only the reduction of pollen germination but also the reduction of tube growth. Also, NOS inhibitor NG-nitro-L-Arg-methyl eater (L-NAME ), NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3- oxide(c-PTIO) and two scavengers of H2O2, ascorbic acid (ASC) and catalase (CAT) not only largely prevented eATP-inhibited pollen germination but also tube growth. These results indicated that NO and H2O2 are involved in eATP inhibited pollen germination and tube growth.