Paper Title:
Distinguishing Bacteriolytic from Bacteriostatic Activity of an Antibiotic Agent by Real-Time Quantitative PCR
  Abstract

A simple and rapid real-time quantitative PCR assay was devised to discriminate bacteriolytic from bacteriostatic activity for a given antibacterial agent. Bacteria suspension was incubated with the compound solution, the mixture was centrifuged and supernatant was removed completely, the obtained pellet was then used as the DNA template for PCR. Then the bacteriostatic and bacteriolytic activity can be inferred by the quantity of PCR product. Moreover, the parameters that influence the assay sensitivity was discussed.

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 2: Materials for Biomedical Engineering Application
Edited by
David Wang
Pages
357-360
DOI
10.4028/www.scientific.net/AMR.343-344.357
Citation
Z. P. Wang, "Distinguishing Bacteriolytic from Bacteriostatic Activity of an Antibiotic Agent by Real-Time Quantitative PCR", Advanced Materials Research, Vols. 343-344, pp. 357-360, 2012
Online since
September 2011
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Price
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