Paper Title:
Enzymatic Synthesis of both Enantiomers of Ethyl 4-Cyano-3-Hydroxybutanoate
  Abstract

(R)-Ethyl 4-cyano-3-hydroxybutanoate is the key intermediate for the synthesis of liptor used for lowering high cholesterol and triglycerides. Firstly both enantiomers of ethyl 4-cyano-3-hydroxybu- tanoate were prepared by the enzymatic reduction of ethyl 4-cyano-3-oxobutanoate with two whole cells in high ee and high yield. The (R)-enantiomer was obtained in 89.8% yield and 98.5% ee in the catalysis of 20 mM substrate by the strain Bacillus pumilus Phe-C3; the (S)-enantiomer was achieved in 83.1% yield and 95.4% ee in the catalysis of 10 mM substrate by the strain Klebsiella pneumomiae Phe-E4. Preparative synthesis were carried out on 200-L scale.

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 2: Materials for Biomedical Engineering Application
Edited by
David Wang
Pages
448-452
DOI
10.4028/www.scientific.net/AMR.343-344.448
Citation
J. Z. Jin, J. Zhang, "Enzymatic Synthesis of both Enantiomers of Ethyl 4-Cyano-3-Hydroxybutanoate", Advanced Materials Research, Vols. 343-344, pp. 448-452, 2012
Online since
September 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Zhao Xiang Han, Zhang Meng, Chun Xia Lv
Chapter 10: Chemistry Science and Applied Chemistry
Abstract:Abstract. This study discussed the effect factors of biodegradation on phenanthrene by P.putida. The outcomes showed that the optimal...
2107
Authors: Shu Xu
Chapter 2: Biology Technology and Materials
Abstract:L-phenylalanine fermentation (L-Phe) is produced by the preparation method of PPA-transamination using the ferment liquor, which is the...
742
Authors: Lu Yu, Shu Hong Mao, Shao Xian Ji, Fu Ping Lu
Chapter 11: Biological and Environment-Friendly Materials
Abstract:Dehydrogenated steroids are usually more effective in treating diseases, compared to their precursors. In this study, toxicity of six ionic...
719