Paper Title:
Highly Enantioselective Hydrolysis of Racemic Isopropyl Tert-Leucinate by Newly Discovered Baclicus Lincheniformis Jx010 for Synthesis of L-Tert-Butyl Leucine
  Abstract

Newly discovered strain Baclicus lincheniformis JX010 was identified to enantioselective hydrolysis of racemic ethyl tert-leucinate for the synthesis of chiral L-tert-butyl leucine. In the hydrolysis of isopropyl tert-butyl leucinate, the L-tert-butyl leucine was synthesized in 99% ee and 48% conversion. The cells was immobilized on synthetic resin 0501 without pretreatment to increase the enzyme stability. A series of organic cosolvents were investigated the hydrolysis rate and 2% glycerol was considered as the optimized cosolvent. In the hydrolysis of 50 mM isopropyl tert-butyl leucinate, the immobilized cells remained 85%activity with L-tert-butyl leucine in 99% ee after 8 reaction cycles.

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 2: Materials for Biomedical Engineering Application
Edited by
David Wang
Pages
453-456
DOI
10.4028/www.scientific.net/AMR.343-344.453
Citation
J. Z. Jin, J. Zhang, "Highly Enantioselective Hydrolysis of Racemic Isopropyl Tert-Leucinate by Newly Discovered Baclicus Lincheniformis Jx010 for Synthesis of L-Tert-Butyl Leucine", Advanced Materials Research, Vols. 343-344, pp. 453-456, 2012
Online since
September 2011
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Price
$32.00
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