Paper Title:
Strain Improvement of Acinetobacter Calcoaceticus D29 for Enhanced Lipase Production by Screening Sodium Citrate Resistant Mutants
  Abstract

A strain improvement program was developed to increase lipase production by Acinetobacter calcoaceticus D29, in which mutants were generated by Ultraviolet (UV) radiation and selected by resistance to 1.5% (w/v) sodium citrate. One of the mutant strains UN9 exhibited 48.69% more lipase activity than the original strain did. Although the UV random mutagenesis alone is an effective method for general strain improvement, our results showed that UV mutagenesis combined with selection for sodium citrate resistance is a more efficient strategy for screening mutant strains with enhanced lipase production.

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 2: Materials for Biomedical Engineering Application
Edited by
David Wang
Pages
551-555
DOI
10.4028/www.scientific.net/AMR.343-344.551
Citation
S. J. Zhong, W. Qi, H. K. Wang, "Strain Improvement of Acinetobacter Calcoaceticus D29 for Enhanced Lipase Production by Screening Sodium Citrate Resistant Mutants", Advanced Materials Research, Vols. 343-344, pp. 551-555, 2012
Online since
September 2011
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Price
$32.00
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