Paper Title:
Research on the Characteristics and Culture Conditions of Saccharomyces boulardii
  Abstract

The morphological, physiological and biochemical characteristics of Saccharomyces boulardii were studied for the preparation of highly active yeast. The optimal condition of the flask culture including inoculum size, culture temperature, pH value, were investigated separately. The results showed that S.boulardii was asporogenous and had better heat tolerance and acid tolerance growing well at 37°C and pH 2.0. We also found that thermal death temperature was 55~56°C and S.boulardii is well ethanol-tolerant, for the highest concentration of ethanol that S.boulardii can tolerate was 20%. The optimal culture conditions in a flask culture was YEPD medium with 50ml/250ml medium volume, natural pH, 5% inoculum size, 32°C culture temperature, 160 r/min shaker speed, and 24 hours cultivation period. Under these conditions, suspension was diluted 10 times and the optical density at 600 nm was up to 0.85.

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 2: Materials for Biomedical Engineering Application
Edited by
David Wang
Pages
594-598
DOI
10.4028/www.scientific.net/AMR.343-344.594
Citation
L. P. Du, R. X. Hao, D. G. Xiao, L. L. Guo, W. D. Gai, "Research on the Characteristics and Culture Conditions of Saccharomyces boulardii ", Advanced Materials Research, Vols. 343-344, pp. 594-598, 2012
Online since
September 2011
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Price
$32.00
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