Paper Title:
Effects of Daidzein or Genistein on Proliferation and Antioxidation of Mammary Epithelial Cell of Dairy Cow In Vitro
  Abstract

Effects of Daidzein or Genistein on the proliferation and antioxidation of mammary epithelial cells of dairy cow were investigated in vitro. 10 groups were assigned including blank control, estradiol (E2) group(10 ng/mL), different concentrations of Daidzein (1, 10, 100, 1 000 ng/mL) and Genistein (1, 10, 100, 1 000 ng/mL) groups. The MMT method was used to determine the proliferation effect of Genistein or Daidzein, and the results showed that Genistein at the concentration of 10 and 100 ng/mL, and Daidzein at the concentration of 100 and 1000 ng/mL significantly improved dairy cow mammary epithelial cell proliferation (P<0.05) , while significantly weaker than E2 group(P<0.05). In the antioxidation experiment, the T-SOD and GSH-PX activity, MDA and NO content of the mammary epithelial cells at the logarithmic growth phase treated with Daidzein or Genistein for 24 h were measured and the results showed that 100, 1000 ng/mL Daidzein, and 100, 1000 ng/ mL Genistein significantly increased the T-SOD activities and decreased MDA content (P<0.05).1 000 ng/ mL Daidzein and 100, 1 000 ng/mL Genistein significantly increased the GSH-PX activites (P<0.05). The results showed that proper levels of daidzein and genistein can improv the proliferation and antioxidation function of mammary epithelial cell of dairy cows.

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 2: Materials for Biomedical Engineering Application
Edited by
David Wang
Pages
649-654
DOI
10.4028/www.scientific.net/AMR.343-344.649
Citation
C. L. Liu, Z. Q. Li, X. J. Feng, "Effects of Daidzein or Genistein on Proliferation and Antioxidation of Mammary Epithelial Cell of Dairy Cow In Vitro", Advanced Materials Research, Vols. 343-344, pp. 649-654, 2012
Online since
September 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Shu Feng Li, Li Hao, Hui Li Tong, Dan Dan Zhao, Yun Qin Yan
New Functional Materials
Abstract:To drink excessively for long time would damage human genital system seriously, nevertheless there is still less researches on the effect of...
116
Authors: Xiao Yan Xie, Li Li Xu, Dong Mei Gao
Session 1: Materials for Environmental Protection and Energy Application
Abstract:This study was conducted to explore the effect of CuO nanoparticles (NPs) on the cell membrane permeability and its exclusion from cells....
77
Authors: Yu Ping Li, Hu Su, Xiao Fang Pi, Yan Chun Gong, Xiang Yuan Xiong, Guang Jie Wu, Zi Ling Li
Chapter 10: Chemical Biology and Medicinal Chemistry
Abstract:The medicinal plant Scutellaria baicalensis Georgi has been used widely in traditional Chinese medicine for anti-inflammation, anticancer,...
1673
Authors: Jun Yang, Chun Chu, Biao Deng, Sai Liang Ding, Guang Hui Wang, Yong Zhang, Zhi Hua Quan
Chapter 1: New Materials and Advanced Materials
Abstract:Abstract Objective To testify the special cytotoxicity of TGF-alpha-SAP on proliferating vascular smooth muscle cells and endothelial cells....
182
Authors: Shu Jing Wang, Fei Wang, Jia Liu, Shan Jiang, Ning Chen, Lin Liu, Ying Zhao, Xiao Dan Zhang
Chapter 3: Biochemical, Medicine Engineering and Technologies, Applications of Genetic Engineering
Abstract:The direct anti-tumor T-7 peptide of tumstatin was obtained to study its effect on human hepatoma cells (HepG-2) and human umbilical vein...
744