Nowadays, the immobilization of At.t is a research potential. The immobilization of At.t on the sawdust filler was discussed in this paper. The experimental results indicated that sawdusts, as the immobilization fillers, can provide a stable living microenvironment for At.t showing the favorable bioactivity and strong acid-producing capacity. Also, under the optimal conditions with the inoculums concentration 12.5%, sulphur dosage 1% (w/v), dissolved oxygen (DO) in the bioreactor above 6 mg/l, the conductivity, pH, and H+ in the bioreactor were 45.2 ms/cm，0.964, and 0.109 mol/l，respectively. In the leaching ore experiment within 24 hours, bacteria effect of phosphorus removal was unconspicuous.