This research aimed to study the effects of 30 kinds of Chinese herbs on fungi in food. 30 kinds of Chinese herbs and 6 common fungi in food were used as experiment material. The effects of water, ethanol absolute and petroleum ether extracts on fungi were studied by filter paper method. The results showed that water extracts of Coptis chinensis. and Magnolia officinalis Rehd.et Wils., the ethanol extracts of Syzygium aromaticum Thunb., Coptis chinensis, Cinnamomum cassia Presl and Cassia Bark, the petroleum ether extracts of Syzygium aromaticum Thunb. and Cassia Bark had strong antimicrobial ability. The study can provide the scientific basis for exploring natural food preservatives.