Paper Title:
Construction and Vaccine Studies on a Pseudorabies Virus with Deletions in Glycoprotein I and Glycoprotein E Genes
  Abstract

A pseudorabies virus (PRV) mutant with deletions in genes for glycoprotein I (gI) and glycoprotein E (gE) was constructed and evaluated as a candidate vaccine strain for its protective by virulent wildtype (wt) PRV Ea strain challenge in pigs. Transfer plasmid pIESE was constructed and co-transfected with PRV genome into IBRS-2 cells to generate a recombinant mutant PRV Ea gI-/gE- virus. The recombinant virus was confirmed by Southern blotting and indirect immunofluorescence assay (IFA). In animal experiments, the immunogenicity was tested by PRV-enzyme-linked immunosorbent assay (ELISA) and PRV neutralizing assay. PRV Ea gI-/gE- elicited significant humoral immune responses to wt PRV Ea, and PRV gI-/gE- immunization protected pigs against a lethal challenge by virulent PRV Ea strain. These results suggested that the recombinant PRV Ea gI-/gE- might be considered as a potential candidate vaccine against PRV.

  Info
Periodical
Advanced Materials Research (Volumes 343-344)
Chapter
Session 3: Biology-Related Materials Research in Environmental Protection
Edited by
David Wang
Pages
868-874
DOI
10.4028/www.scientific.net/AMR.343-344.868
Citation
S. L. Zhang, M. L. Jin, H. C. Chen, "Construction and Vaccine Studies on a Pseudorabies Virus with Deletions in Glycoprotein I and Glycoprotein E Genes", Advanced Materials Research, Vols. 343-344, pp. 868-874, 2012
Online since
September 2011
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