The aim of this study was to select CLA producing bacteria from fermented diary products and human faeces, then evaluate their resistance to simulated gastrointestinal stress and adhesiveness to human intestinal epithelial cells according typically assess methods. Additionally, hydrophobicity, resistance to opportunistic pathogens and phenol ability were analysed. Results showed that three L. paracasei subp. Paracasei (F0721, IN 5.22 and Q9), two Lactobacillus acidophilus (F0221 and M6) and one Lactobacillus plantarum (G14) exert the ability to product conjugated linoleic aicd ranging from 77.21 to 111.59 μg/ml in the presence of 0.5 mg/ml linoleic acid (LA) in MRSc broth under anaerobic conditions at 37°C for 40 h. Strain F0721, IN5.22 and G14 showed higher resistance to gastric acid than other strains at pH 2.0, F0721 and IN5.22 showed higher tolerance ability at pH 3.0. F0221 showed the highest bile salt tolerance than other strains at 0.15%, 0.3% and 0.5% bile concentrations, whereas highest sensitivity to the bile stress was found for IN5.22. F0221, IN5.22 and G14 strains showed the highest levels of adherences (range from 106 to 129 cfu/100 cells), and intestine origin bacteria (F0221, F0721 and IN5.22) exhibited higher hydrophobicity values and resistant to phenol ability as compared to the dairy products.