.In this study, the nutrient components of the wild and the liquid suspension cultured Nostoc flagelliforme were determined and compared by atomic absorption method, HPLC and so on. The results indicated that the contents of crude proteins, crude fat, β-carotenes, vitamin C and mineral elements in N. flagelliforme cells from the suspension culture were higher than those in wild ones. 17 kinds of amino acids have been measured and the data showed that the contents of 13 kinds of them in cultured cells were higher than those in wild ones except aspartic acid, valine, isoleucine and arginine. Antitumor effect of the extracellular polysaccharides indicated that the extracellular polysaccharides could inhibit the growth of cervical carcinoma Hela cells. Their inhibition rates are 32.22% and 37.32%, respectively. This investigation showed that the liquid suspension cultivated N. flagellifome can be a substitute for the wild algae to be used as functional food resource.