Paper Title:
A Scale up Submerging Fermentation to Produce Fibrinolytic Enzyme and Mycelia by Cordyceps militaris
  Abstract

A 10 and 100 L fermentor was used to produce Fibrinolytic enzyme and mycelia by Cordyceps militaris, which was based on the optimized results of the flasks ferment of submerging fermentation and with the principle of equivalent KLa. Two operating parameters of the ferment, stirring speed and ventilation were determined. The results showed that, under the identical KLa with flasks, the stirring speed and ventilation were 50 r/min and 700 L/h, 100 r/min and 600 L/h, or 150 r/min and 500 L/h in 10 L fermentor; And the stirring speed and ventilation were 100r/min and 3 m3/h or 150 r/min and 2 m3/h in 100 L fermentor. In the experiments, the stirring speed and ventilation were 100 r/min and 600 L/h in 10 L fermentor; the stirring speed was 250 r/min and ventilation was 2 m3/h in 100 L fermentor, the results were consistent with the optimized conditions in flasks, and the production of both fibrinolytic enzyme and mycelia reached the optimized results in flasks. Therefore, these results are important for producing fibrinolytic enzyme and mycelia by a scale-up of Cordyceps militaris fermentation.

  Info
Periodical
Chapter
Chapter 2: Smart Materials for Biomedical Engineering Application
Edited by
J.L. Zhong
Pages
239-244
DOI
10.4028/www.scientific.net/AMR.345.239
Citation
J. H. Guo, H. X. Chen, X. L. Liu, H. Gao, Y. P. Deng, J. Xiao, J. K. Zhang, "A Scale up Submerging Fermentation to Produce Fibrinolytic Enzyme and Mycelia by Cordyceps militaris", Advanced Materials Research, Vol. 345, pp. 239-244, 2012
Online since
September 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Li Li Feng, Jian Fei Zhang, Hui Luo, Zheng Li, Hong Jie Zhang
Abstract:The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture...
192
Authors: Zhong Xu, Nan Liu, Dan Zhao, Duo Wang, Chun Bo Che, Ping Yang, Li Kun Huang
Environmental Biotechnology
Abstract:The influencing factors of potato starch liquefaction system which use the α-amylase to hydrolysis, the degree of hydrolysis of starch (DE)...
1278
Authors: Hua Zheng Liang, He Chen, Jian Feng Wang, Yu Lan He
Abstract:Co-immobilize enzyme by cross-linking and embedding, optimize conditions for immobilizing, determinate the enzymatic properties of...
1793
Authors: Peng Li, Wei Guo Wang, Yu Jia Cui, Yong Liang Zhao, Ya Nan Gu
Chapter 7: Biochemical Industry
Abstract:Purpose To research out a suitable method for the determination of enzymatic activity for β-glucan synthase. Methods According to the methods...
1583
Authors: Dan Huang, Zhi Chao Shang, Yu Long Li, Yu Long Chu, Xian Ling Yuan
Chapter 8: Biological Chemical and Technology
Abstract:A Rhizopus strain producing esterifying enzyme was isolated from Luzhou-flavor DaQu. It was preliminary identified as Rhizopus arrhizus....
1229