The hypolipidemic effect and anti-oxidative activity of seabuckthorn marc flavonoids (SMF) on hyperlipidemic rats was investigated. The rats were divided into 5 groups, including normal control group, hyperlipidemic model control group and 3 different doses of SMF groups i.e. 3.0 mg/kg·bw, 30.0 mg/kg·bw, 90mg/kg·bw, respectively. The SMF was given orally for 35 days. After the treatment, the body, fat and liver weights of rats were weighed. The content of serum total triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and the activity of lipid metabolism and anti-oxidative enzyme were determined. The levels of serum lipids in hyperlipidemic rats fed with SMF were significantly lower than model control group. The activities of hepatic lipase (HL), lipoprotein lipase (LPL), lipidase (LPS), superoxide dismutase (SOD), glutathione (GSH) in liver were increased, while the contents of malondialdehyde (MDA) were decreased in SMF groups. The results indicate that SMF has significantly hypolipidemic effects and antioxidant activity.