Paper Title:
Novel Approach to Fabricate an Amperometric Biosensor for Glucose Based on Nafion, Azure І and Gold Nanoparticles
  Abstract

A novel approach to fabricate an amperometric biosensor for the detection of glucose was described. By means of self-assembled technique and the opposite-charged adsorption, Nafion(Naf), Azure І (Azu), gold nanoparticles (nano-Au) and glucose oxidase (GOD) were immobilized on the gold electrode subsequently. The GOD immobilized on the nano-Au monolayer was stable and retained its functional activity. The stepwise self-assemble procedure of the immunosensor was further characterized by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The factors influencing the performance of the resulting biosensor were studied in detail. The biosensor could detect the glucose in a range of 5.1×10-6~4.0×10-3mol/L.

  Info
Periodical
Chapter
Chapter 2: Smart Materials for Biomedical Engineering Application
Edited by
J.L. Zhong
Pages
331-333
DOI
10.4028/www.scientific.net/AMR.345.331
Citation
Y. Zhou, "Novel Approach to Fabricate an Amperometric Biosensor for Glucose Based on Nafion, Azure І and Gold Nanoparticles", Advanced Materials Research, Vol. 345, pp. 331-333, 2012
Online since
September 2011
Authors
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Price
$32.00
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