.Purposes,To explore the effects of carnosine on high glucose-induced apoptosis of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro. The cellular apoptotic model was made by the addition of high glucose (25 mmol/L), the group of high glucose and carnosine was administered by the addition of high glucose (25 mmol/L) with carnosine (20 mmol/L). In addition, cell apoptosis was detected by the electron microscopy and AnnexinV/PI flow cytometry. Results Compared with the control group, high glucose could induce HUVECs apoptosis under electron microscopy and AnnexinV/PI flow cytometry, while 20 mmol/L carnosine could inhibit the apoptosis induced by high glucose significantly (##P <0.05). Conclusion In this study, carnosine could inhibit high-glucose induced apoptosis of human umbilical vein endothelial cells.