Paper Title:
Double-Conjugated Ru(bpy)32+ Complexes for Enhanced Electrochemiluminescence Immunoanalysis
  Abstract

A new method was established to construct a ultrasensitive electrochemiluminescence (ECL) immunosensor by using Ru@SiO2 (Ru(bpy)3 2+ doped silica) - doped Au (Ru@SiO2-Au) composite nanoparticles to label Ru-Ab2 (monoclonal α-fetoprotein (AFP) antibody labeled with Ru(bpy)32+). The primary antibody, anti-AFP was first immobilized on the multi-walled carbon nanotubes (MCNTs) modified with gold nanoparticles (MCNTs-Au) modified glassy carbon electrode due to the covalent conjugation, then the antigen and the Ru@SiO2-Au composite nanoparticles labeled Ru-Ab2 (Ru@SiO2-Au~Ru-Ab2)was conjugated successively to form a double-conjugated Ru(bpy)32+ sandwich-type immunocomplex through the specific interaction. Accordingly, through the ECL response of Ru@SiO2-Au~Ru-Ab2 and tripropylamine (TPA), a strong ECL signal was obtained and an amplification analysis of protein interaction was achieved. The present immunosensor performed high sensitivity and wide liner for detection α-fetoprotein (AFP) in the range of 0.05-80 ng mL−1 and the limit detection was 0.025 ng mL−1(defined as S/N=3). The morphologies of MCNTs, MCNTs-Au, Ru@SiO2 and Ru@SiO2-Au nanoparticles were characterized by using transmission electronic microscopy (TEM). The developed label and method is versatile, offers enhanced performances, and can be easily extended to other protein detection.

  Info
Periodical
Chapter
Chapter 2: Smart Materials for Biomedical Engineering Application
Edited by
J.L. Zhong
Pages
381-386
DOI
10.4028/www.scientific.net/AMR.345.381
Citation
J. G. Hou, F. T. Hu, Y. T. Cao, H. J. Jin, T. H. Li, N. Gan, "Double-Conjugated Ru(bpy)32+ Complexes for Enhanced Electrochemiluminescence Immunoanalysis", Advanced Materials Research, Vol. 345, pp. 381-386, 2012
Online since
September 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: H.J. Lee, M.A. Do, E.J. Kim, J.H. Yeum, H.D. Ghim, Jin Hyun Choi
Abstract:Ultra-small and well dispersed silver nanoparticles were prepared by mixing AgNO3 in PEI (polyethylenimine) solution. Particle size analysis...
1080
Authors: Hyoung Bong Bae, Jung Ho Ryu, Bok Soo Byun, Seong Ho Choi, Sang Ho Kim, Chul Gyun Hwang
Abstract:Pt-Ru@CP-MWNT catalysts were prepared by radiolytic deposition of Pt-Ru nanoparticles on conduction polymer (CP) coated multi walled carbon...
1478
Authors: Ana Karina Cuentas-Gallegos, Abraham Zamudio-Flores, Montse Casas-Cabanas
Abstract:Nanocomposite hybrid materials (NHM) based on the immobilization and dispersion of SiW12 polyanion (H4SiW12O40) on a highly oxidized...
11
Authors: Thidarat Wangkam, Kitti Boonperm, Phatra Khomkrachang, Toemsak Srikhirin, Verayuth Praphanphoj, Boonsong Sutapan, Armote Somboonkaew, Ratthasart Amarit
Chapter 2: Nanomaterials and Nanotechnologies in Biomedical Application
Abstract:Hepatocellular carcinoma is a one of the most common cancer in the world and the third cause of cancer-related death. The recommended...
84