Paper Title:
Analysis for Effect of Dimehypo on Proteolytic Enzyme Activity in the Midgut of the Spider Pardosa pseudoannulata with a Piezoelectric Quartz Crystal Impedance
  Abstract

A piezoelectric quartz crystal impedance technique has been applied for in situ monitoring of the whole process of the proteolytic enzyme-catalyzed hydrolyzation of casein and the effect of pesticide dimehypo (formula: C5H11NNa2O6S4) on the proteolytic enzyme activity in the midgut of the spider Pardosa pseudoannulata, based on its real-time reponses to the density-viscosity change of the tested solution due to the hydrolyzation occurring on the casein molecules. The results indicated that the hydrolyzation was significantly increased in the presence of low-dose dimehypo, where significantly inhibited in the presence of high-dose dimehypo, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of dimehypo, with a significant frequency response observed even at a 2.88 x 10-4 volume ratio of dimehypo to distilled water. The present work may have presented a novel method feasible for study on the mechanism of excitability of spiders under low-dose dimehypo pesticide and quick detection of proteolytic enzyme activity.

  Info
Periodical
Chapter
Chapter 2: Smart Materials for Biomedical Engineering Application
Edited by
J.L. Zhong
Pages
387-392
DOI
10.4028/www.scientific.net/AMR.345.387
Citation
W. H. Fu, J. Fang, Z. Wang, "Analysis for Effect of Dimehypo on Proteolytic Enzyme Activity in the Midgut of the Spider Pardosa pseudoannulata with a Piezoelectric Quartz Crystal Impedance", Advanced Materials Research, Vol. 345, pp. 387-392, 2012
Online since
September 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Hong Yu Liang, Mei Li, Ming Shi, An Ping Liao, Ru Chun Wu
Chapter 1: Advanced Processing Technology
Abstract:In order to prevent the activity loss of bromelain activity in the production process, the effect of temperature, pH value and metal ions on...
19
Authors: Qiu Dong Pan, Ming Xia
Chapter 6: Environmental Chemistry, Environmental Engineering and Environmental Protection
Abstract:Box-Behnken experiment design was used to optimize the enzymolysis technology of casein, and the antibacterial activity of casein peptides...
977
Authors: Zhen Yan Liu, Wen Qin Yang, Han Liu, Jie Zhang, Chuan Gang Zang, Yu Chao Liu, Hong Guan
Chapter 3: Biotechnologies and Microbiology, Pharmacology, Biomaterials and Tissues
Abstract:In order to study the ability of Lactobacillus bulgaricus hydrolyzed protein, using casein sodium as substrate, the content of free...
535