Paper Title:
The Study on the Interaction Conditions of Water-Soluble Chitosan with Bovine Serum Albumin
  Abstract

The interaction conditions between the water-soluble chitosans(WSC) and bovine serum albumin (BSA) was studied by Ultraviolet-visible absorption and fluorescence spectrometries. It was shown there was a good linear relationship between the absorbency A and the BSA concentration(0~1.5g/L) and WSC concentration (0~1.5 g/L). The fluorescence quenching of WSC to BSA was static quenching. When the temperature is 30°C, its binding constant Ka=5.35×104 L/mol, binding site n=1.05. The influence of WSC on the conformation of BSA was analyzed by sychronous fluorescence spectra and three-dimensional fluorescence spectrum.

  Info
Periodical
Advanced Materials Research (Volumes 347-353)
Chapter
Chapter 1: Development and Utilization of Solar Energy
Edited by
Weiguo Pan, Jianxing Ren and Yongguang Li
Pages
1281-1286
DOI
10.4028/www.scientific.net/AMR.347-353.1281
Citation
S. S. Huang, F. Z. Qu, T. K. Xu, L. Cui, "The Study on the Interaction Conditions of Water-Soluble Chitosan with Bovine Serum Albumin", Advanced Materials Research, Vols. 347-353, pp. 1281-1286, 2012
Online since
October 2011
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Price
$32.00
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