Paper Title:
Construction of Non-Antibiotic Resistance Expression Vector by Secretory β-Gal as a Selective Marker in Lactobacillus
  Abstract

On account of the spreading problem of resistance gene and off-target effects of functional gene products expressed by bacteria, pMG36e, a plasmid with the erythromycin- resistance (Emr) gene, which could shuttle between Lactobacillus and E. coli, was reconstructed into a non-antibiotic secretory expression vector with the beta-Galactosidase (β-Gal) gene for selection, then linked a secretory signal peptide sequence to the non-antibiotic resistance vector, thus the secretory non-antibiotic resistance vector was constructed. Finally, to test the vector's function, the SO7 gene of Eimeria tenella (E. tenella) was cloned into the vector and expressed successfully. This system may be used as a model to explore the feasibility of vaccine vectors using the β-Gal gene for selection, and collect fundamental data for expressing other homogeneous and heterogeneous functional genes in such a novel vector.

  Info
Periodical
Advanced Materials Research (Volumes 347-353)
Chapter
Chapter 1: Development and Utilization of Solar Energy
Edited by
Weiguo Pan, Jianxing Ren and Yongguang Li
Pages
269-276
DOI
10.4028/www.scientific.net/AMR.347-353.269
Citation
Z. Sun, H. F. Zhang, M. Wang, "Construction of Non-Antibiotic Resistance Expression Vector by Secretory β-Gal as a Selective Marker in Lactobacillus", Advanced Materials Research, Vols. 347-353, pp. 269-276, 2012
Online since
October 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Ai Li, Guo Xing Yang, Wei Zhang
Abstract:To establish a rapid, sensitive and specific multiplex PCR method for the simultaneous detection of Staphylococcus aureus,...
2803
Authors: Wan Li, Xiu Hong Xu, Jia Lei Xiao, Bi Xian Zhang, Hong Tao Li, Yong Cai Lai
Chapter 7: Pollution Control Project (Air, Water, Solid)
Abstract:Like bacteria, fungi play an important role in the composting process as major decomposers of organic substances. As only a small fraction of...
1747
Authors: Yi Shu Yang, Xiao Na Sun, Di Liu, Xiao Li Wang, Si Si Shen, Ze Lin Li, Yi Zeng
Chapter 2:Materials Science and Bioengineering
Abstract:Novel targets against HIV-1 are booming recently. APOBEC3G has the potential to inhibit the replication of HIV-1, while its antiviral...
141
Authors: Xiao Hua Yang, Xiao Feng Wei, Jian Hua Hu, Xiong Wei Liu, Shao Jin Chen
Chapter 2: Biology Technology and Materials
Abstract:Swine and canine influenza, which is acute viral respiratory infectious disease, can do great threat to the health of people and animals....
733
Authors: Yu Fen Jin, Yan Lei Li, Yan Hua, Xiao Gang Zhang, Ting Yu
Chapter 6: Biological Chemistry, Pharmaceutical, Medicine and Biomedical Engineering
Abstract:Objective To evaluate the effectiveness of prokaryotic expression of fusion proteins pp150-pp65 of human cytomegalovirus (hCMV) for its...
1313