Paper Title:
Applications of Reversed-Phase High-Performance Liquid Chromatoraphy in Strains Identification of the Bean Sprout
  Abstract

Lactic acid which was fermented by bean sprout was determined and the lactobacillus in bean sprout was identified by High-performance Liquid Chromatoraphy(HPLC). The optimal HPLC chromatographic conditions were determined as follows:Agilent XDB-C18 chromatography column with UV detection at 210nm.The mobile phase was Phosphate buffer solution(pH=2.3):methanol:acetonitrile(95%:2%:3%).The flow rate was 0.5ml/min.This method is accurate,Speedily and reproducible. This method can identify the lactobacillus, and gives a qualitative and quantitative analysis of the lactic acid which was the metabolites of Yibin bean sprout.

  Info
Periodical
Advanced Materials Research (Volumes 347-353)
Chapter
Chapter 1: Development and Utilization of Solar Energy
Edited by
Weiguo Pan, Jianxing Ren and Yongguang Li
Pages
354-359
DOI
10.4028/www.scientific.net/AMR.347-353.354
Citation
Y. Zuo, S. Ju, Y. Li, H. Xie, L. P. Liu, F. M. Wei, A. G. Liu, "Applications of Reversed-Phase High-Performance Liquid Chromatoraphy in Strains Identification of the Bean Sprout", Advanced Materials Research, Vols. 347-353, pp. 354-359, 2012
Online since
October 2011
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Price
$32.00
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