Paper Title:
Optimizing Immobilization of Horseradish Peroxidase in the Nano-Scale Porous Structural Inorganic Material
  Abstract

Horseradish peroxidase was immobilized by montmorillonite to construct a nano-scale structural biocatalyst for the stabilization and/or detoxification of phenolic compounds from natural waters. Humic substances extracted from soils were used as a bonding agent, stimulating peptide covalent bonds between enzymes and surface of inorganic clay minerals. Operating conditions including concentrations of enzyme inoculated and duration time and temperature for the immobilization were examined and the optimal condition for maximizing the quantity of enzyme immobilized was found. The results of this study are expected to provide the essential information for the process-efficient and economic enzyme immobilization operations in the environmental engineering as well as for establishing ecological green U-City.

  Info
Periodical
Advanced Materials Research (Volumes 356-360)
Chapter
Chapter 6: Environmental Engineering
Edited by
Hexing Li, Qunjie Xu and Daquan Zhang
Pages
1085-1088
DOI
10.4028/www.scientific.net/AMR.356-360.1085
Citation
J. A. Kim, J. S. Lim, H. S. Kim, "Optimizing Immobilization of Horseradish Peroxidase in the Nano-Scale Porous Structural Inorganic Material", Advanced Materials Research, Vols. 356-360, pp. 1085-1088, 2012
Online since
October 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Jie Zhang, Yang Yu, Jing Ren, Bin Song Wang
Abstract:Macroporous exchange resins have been applied as supports for enzyme immobilization. The supporters were activated by cross-linking with...
2115
Authors: Rui Jing Su, Wen Feng Zhang, Peng Hui Shi, Min Cong Zhu, Deng Xin Li
Chapter 1: Mechatronics and Automation
Abstract:Graphene oxide was used as substrate for immobilizing alkaline protease. The immobilizing conditions were optimized though single-factor test...
15
Authors: Feng Zhang
Chapter 7: Building Materials
Abstract:Zinc oxide nanoparticles was synthesized by microwave decomposition of zinc acetate precursor. Horseradish peroxidase (HRP) was immobilized...
1608
Authors: Cheng Zou, You Yan Liu, Yi Ming Qin, Ai Xing Tang, Li Na Lan
Chapter 6: Biological Chemistry, Pharmaceutical, Medicine and Biomedical Engineering
Abstract:The synthesis of α-pinene oxide mediated by Novozym 435 (lipase from Candida antarctica) in a three-phase system was studied in this work....
896
Authors: Sai Nan Xu, Yong Chen
Chapter 3: Photocatalytic Treatment, Degradation and Recycling of Pollutants and Wastes
Abstract:A novel method was developed for degradation of phenolic waste water using horseradish peroxidase (HRP) immobilization on activated...
256