Hydrogen sulfide (H2S) is a malodorous gas which can cause nuisance and health effects to people who live and work in the contaminated air environment. Therefore, the main objective of this work was to study the removal of hydrogen sulfide (H2S) using a biofilter packed with mixture of compost, organic fertilizer, wood chips and municipal wastewater sludge at a ratio of 60:10:20:10 by weight. The experiment was carried out at hydrogen sulfide concentration of 5-200 ppmv and empty bed residence time (EBRT) of 60 seconds. The results indicated that completer H2S removal can be achieved at H2S inlet concentration of 200 ppmv. Elimination capacity of the biofilter reached as high as 24.38 gm−3 h−1.