In Hainan ChengMai Kudingcha Plantation, soil, roots, leaves of Ilex kudingcha C. J. Tseng were obtained from 11 sampling points as “S” and analyzed for Cd and physical and chemical properties of soil, then the concentration and mobility of Cd in tea trees-soil was studied. The speciation of Cd in soil were analyzed by BCR three stage sequential extraction procedure and one-step extraction. Method characterized Bioavailability of Cd in Ilex kudingcha C. J. Tseng were established through the correlation analysis of Cd speciation in soil to Cd content in leaves and the influence of physical and chemical properties of soil to Bioavailability of Cd in soil was discussed. Results of this study showed that: The majority of Cd in soil was transferred to plant as the strong enrichment of Ilex kudingcha C. J. Tseng Trees, The Cd content distribution was: roots > leaves >>rhizosphere soil; According to the correlation analysis, （F1）、（F1+F2） and Cd-HCl extraction were used to characterize the Bioavailability of Cd in soil due to complex soil environment. The order of physical and chemical properties of soil which affected Bioavailability of Cd was: pH>Eh>organic matter>moisture>volatile phenol, that is to say, lower pH, higher Eh, organic matter, moisture, volatile phenol was in favor of reducing the Bioavailability of Cd and Cd accumulation in tea.