A series of V2O5/WO3/TiO2 plate catalysts were prepared via the incipient wetness impregnation method, with the V2O5 content of 0.68 wt%, 1.01 wt%, 1.19 wt%, 1.5 wt% and the WO3 loading of 4.8 wt%, 6.2 wt%, 7.9 wt%, 9.2 wt%, respectively. Selective catalytic reduction of nitric oxide by ammonia (NH3-SCR) experiments were performed to investigate the effects of the several factors on the performance of the catalysts, including the V2O5, WO3 and MoO3 content in the catalysts and the O2 concentration in the flue gas. The results indicated that as the rising of the V2O5 content, the SCR activity of the V2O5/WO3/TiO2 catalysts was firstly increased and then slightly decreased, with the best activity obtained at the V2O5 content of 1.19 wt%. As the increasing of the WO3 content, the activity was firstly increased slightly and then almost kept constant. Furthermore, the loading of the MoO3 on the V2O5/WO3/TiO2 plate catalysts would bring negative effects to the catalytic activity. In addition, the capability of the catalysts would slightly increase as the O2 concentration rising up to 7%, and then became stable at higher O2 concentrations.