The micro-emulsion liquid membrane (MELM) was prepared for the extraction of hexavalent chromium (Cr (Ⅵ)) from wastewater. The membrane phase consists of kerosene as a membrane solvent, tributyl phosphate (TBP) as a mobile carrier, and sorbitan monooleate(Span80) as a surfactant. A NaOH solution is the internal aqueous phase. The effects of different conditions on the extractive of Cr (Ⅵ) were discussed. The results showed that the removal rate of Cr (Ⅵ) could reach 99.78% when the NaOH concentration was of 0.1mol•L-1 in internal phase, the pH values 1.0-1.75 in external phase, Rew of 1:3 and Roi of 2:1, stirring time of 12min and stirring rate of 320r•min-1, 10% of carrier and 15%-17% of surfactant.