Domestic waste is easily decomposed by microbes to produce odor detrimental to environmental quality. The production of biofertilizer is one of low environment pollution method for domestic waste treatment. The effect of biofertilizer from domestic waste on Chinese traditional vegetable, pak choi (Brassica chinensis), was investigated. The biofertilizer from domestic waste can improve output with 79.7% and increase the chlorophyll content of pak choi. The biofertilizer treatment with more than 100 kg/hm2 can increase phosphorous accumulation and the treatment with 1000 kg/hm2 has the highest potassium accumulation in the above-ground fraction of pak choi. The biofertilizer at 1000 kg/hm2 level has the similar effects for absorption of nutritional elements of N, P and K with chemical fertilizer. The biofertilizer, especially at 1000 kg/hm2 level, can improve the organic matter index of soil.