In this study, the GFP transplastomic tobacco (TG) and non-transgenetic (WT) were compared to study the effects of GFP transplastomic tobacco on soil enzyme activities. The activities of some soil enzymes (urease, cellulase, protease, and invertase) were determined at seeding stage, vegetative stage, flowering stage, and senescing stage. The results showed that there was no significant difference on the activities of urease, cellulase, protease, and invertase between GFP transplastomic tobacco and non-transgenetic at same growing stage. No matter transplastpmic tobacco or controls, the soil enzyme activities of the vegetative stage and flowering stage were higher than seeding stage and senescing stage. The results suggest that the effects of GFP transplastomic tobacco to major soil enzyme activities were not significant.