Petroleum is the most important energy for economic development in China and other countries. But in the process of mining, storage and producing, it has caused many aspects of pollution. In this study, a bacterial strain A-3 which could use ACC as the sole nitrogen sources was isolated from petroleum-contaminated soil. The strain was identified as Achromobacter, according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequence. Strain Achromobacter A-3 could produce ACC deaminase, and could be used for remediation of petroleum-contaminated areas in future.