Recalcitrant and toxic organic pollutants such as aniline from numerous industrial wastewaters can not be efficiently removed using the conventional methods. This study reported a concept for mineralization of aniline in an anoxic reactor, where enhanced biodegradation of aniline were achieved under anoxic conditions. The results indicated that with the presence of nitrate, the degradation rate of aniline was greatly improved compared with the absence of nitrate. From the UV-vis adsorption spectra, COD analysis and denitrification performance analysis, it could be inferred that the cleavage of benzene ring of aniline occurred, aniline could be mineralization by microorganisms under the anoxic condition. However, aniline removal rate was lower compared to aerobic degradation process, and thus needs a significant improvement.