For researching ecological behaviors of Cupriavidus taiwanensis X1 which has strong hydrolysis activity on chlorpyrifos(CP), reporter gene luxAB was successfully introduced into cells by electroporation. The labeled strain X1-lux with genetic stability and fluorescence was obtained. Both of strain X1 and X1-lux could completely degrade 200 mg/l CP within 12h in minimal salt medium, and experimental results showed that introduction of luxAB did not affect strain X1 growth and degradation on CP. The cells of X1-lux and X1 were inoculated into soil with 500 mg/l CP, the cell concentration and CP residual was detected, and data revealed that strain X1 could absolutely removed CP in 12d and survive in soil. Strain X1 is a potential excellent choice for bioremediation of organophosphorus polluted environments.