Paper Title:
Kinetic Studies for the Inhibition Effect of Pentachlorophenol on Alkaline Phosphatase Activity In Vitro
  Abstract

The inhibition kinetic parameters of pentachlorophenol (PCP) on bovine intestinal mucosa alkaline phosphatase (ALP) activity were studied by spectrophotometry. The results showed that PCP inhibited ALP activity in a concentration dependent manner, and the 50% inhibitory concentration (IC50) was estimated to be 4.20 mM. The apparent Michaelis–Menten constant (Km) and apparent maximum reaction rate (Vmax) was found to be decreased in the presence of PCP. Lineweaver–Burk plots indicated that the nature of the inhibition was of an uncompetitive type. Kinetic analysis indicated that the value of the inhibition constant (Ki) was 5.67 mM.

  Info
Periodical
Advanced Materials Research (Volumes 356-360)
Chapter
Chapter 1: Environmental Chemistry and Biology
Edited by
Hexing Li, Qunjie Xu and Daquan Zhang
Pages
309-312
DOI
10.4028/www.scientific.net/AMR.356-360.309
Citation
Q. Y. Liu, "Kinetic Studies for the Inhibition Effect of Pentachlorophenol on Alkaline Phosphatase Activity In Vitro", Advanced Materials Research, Vols. 356-360, pp. 309-312, 2012
Online since
October 2011
Authors
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Takao Saito, Hikoshiro Hayashi, K. Uoe, Takashi Kizuki, Kay Teraoka, Katsuya Kato, Yoshiyuki Yokogawa
Abstract:Our experiments of mouse osteoblast-like MC3T3-E1 cells cultured on a glass substrate showed that as surface roughness of a substrate...
573
Authors: Patricia Valério, Faik N. Oktar, Gültekin Göller, L.S. Ozyegin, A.P.M. Shainberg, Alfredo Goes, M. Fatima Leite
Abstract:Fifteen different lithium-hydroxyapatite composites were tested in this work. LiHA were prepared using 0.25 %, 1 % and 2 % of lithium and...
1121
Authors: Takahisa Anada, Akihiro Araseki, Shou Matsukawa, Tomokazu Yamasaki, Shinji Kamakura, Osamu Suzuki
Abstract:Our previous studies suggested that synthetic octacalcium phosphate (OCP) enhances bone regeneration more than hydroxyapatite (HA). However,...
31
Authors: M.Q. Wang, P. Hou, C.Y. Bao, Jie Weng, Wei Li
Abstract:The purpose of this study was to evaluate the cytotoxicity of nanometer magnetic composite via being cultured with osteoblast-like cells...
1103
Authors: Ying Jie Lei, Ou Yang Jie, Mei Ding
Chapter 3: Chemical and Biomedical Engineering
Abstract:A convenient method to synthesize disodium salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) phenyl phosphate (OXDP), a substrate for the...
1109