Formalin (formaldehyde solution) is generally used as embalming fluid and tissue fixation agent because of its low price, antiseptic sterilization ability and good application effect and so on. However, the intense excitant odor of the formaldehyde and its volatile cause great harm for human health and working environment. In order to solve the problems caused by formaldehyde, an improved non-formaldehyde tissue preservative is invented. The preservative is a blend of acid, buffer solution and cross-linking agent, which act as mildew preventive, stabilizer and fixative respectively. The improved preservative is characterized and applied on animal and body tissue fixation and preservation. The results show that the improved preservative is a more desirable tissue preservative than formaldehyde not only due to its good mildew proof, low volatile and odor, but also the same preservation and fixation effects as formaldehyde. It will be considered as a real environmental friendly preservative in near future.