Because of the relatively narrow space and high density distribution of the passengers, the bus interior environment deteriorates in summer. Natural ventilating introduces a fresh natural freeze, provides the bus interior with appropriate distribution of air supply temperature and velocity field. Making good use of natural ventilation is an operating strategy ideal for improving passengers’ satisfaction, which is considered as an environmental friendly and cost effective approach. Based on CFD numerical simulation, with a whole-domain approach, this paper predicts air flow and thermal comfort in naturally ventilated bus. The outside and inside airflow is modelled simultaneously and within the same computational domain. The thermal environment in different parts of the bus interior is compared. Different vehicle velocities and conditions of windows are taken into account, analysis are made regarding to the effect of both of them on the interior thermal comfort. The result shows, air disturbance at the bus rear parts are intenser than the other parts with better thermal satisfaction; the quality of air flow in different parts makes the temperature difference in bus achieve as high as 3°C; the use of roof openings distributes the temperature more evenly.