The oil-water interfacial tension measurement and enhancing water displacement recovery experiment were carried out, and the effects of various parameters such as category of surfactants, anionic Gemini surfactant concentration, water medium salinity, core permeability, polymer and non-ionic surfactant on anionic Gemini surfactants enhancing water displacement recovery were investigated in detail. The results show that surfactants category is different, its enhancing water flooding recovery efficiency is different, and effect of enhanced oil recovery is consistent with surfactant ability to reduce oil-water interfacial tension. The anionic Gemini surfactant AN12-4-12 is the best in enhancing water flooding recovery efficiency, because it can reduce the oil-water interfacial tension to 5×10-3 mN•m-1. Increasing the concentration of AN12-4-12 is favorable to enhance water displacement recovery. Such as when injecting 0.5PV solution containing 800mg•L-1 AN12-4-12, enhancing water displacement recovery is 11.67%. AN12-4-12 has good adaptability to different salinities (5~25×104 mg•L-1) and low permeability reservoir in improving water displacement recovery. Adding non-ionic surfactant ANT into AN12-4-12 solution can further reduce oil-water interfacial tension and enhance water flooding recovery efficiency. For example, injecting 0.5PV surfactant solution containing 400mg•L-1 AN12-4-12 and 100mg•L-1 can enhance water displacement recovery of 10.7%.