We describe the use of capillary zone electrophoresis (CZE) for the determination of tertiary butylhydroquinone (TBHQ) without derivatization or purification. The influences of buffer pH and voltage on the separation of TBHQ were studied. The internal standard method was used for the quantification of TBHQ. Amperometric detection was carried out at an applied potential of 0.80 V. The detection limit of TBHQ was found to be 10-6 M. Peak intensity varied linearly with TBHQ concentration from 10-4 to 5×10-6 M. The relative standard deviation (RSD) for peak intensity and migration time was in the range of 3.58–4.36% and 0.51–0.94%, respectively. The recovery of the method in food samples is 95.36% for TBHQ. The method developed is suitable for the routine analysis of synthetic phenolic antioxidant TBHQ in samples.