Paper Title:
Optimization and Comparison of Three Methods on Anthocyanins Extraction from Blackcurrant (Ribes nigrum L.) Using RSM
  Abstract

Response surface methodology (RSM) complemented with a central composite design (CCD) was employed to optimize and compare three different anthocyanins extraction methods (solid–liquid extraction(SLE), ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE)) from blackcurrant fruits. The aim was to obtain extracts with high anthocyanins content, which would be potentially interesting for commercial applications as natural colorants. Three major independent variables such as extraction time(min), ethanol concentration (%,v/v), solution to solid ratio (mL/g) were coded at five levels and their actual values were selected on the basis of preliminary experimental results. Results indicated that the yields of anthocyanins varied when extracted with different method, the order of anthocyanins yield from high to low was: MAE (95.77%), UAE (93.65%) and SLE (90.82%). Among three reaction parameters, ethanol concentration consistently tended to significantly affect the anthocyanins yield for above three models (p < 0.0001). MAE was the best of three methods in this study and the optimal values were as follows: extract time controlled at 6.3 min, ethanol concentration maintained at 72.8%, solution to solid ratio equaled to 8.1:1 and working power at 400W. The experimental anthocyanins value under above optimum conditions could reach to 95.77±1.05% that was in perfect agreement with the predicted model 95.52%.

  Info
Periodical
Advanced Materials Research (Volumes 361-363)
Chapter
Chapter 7: Storage and Processing of Agricultural Products
Edited by
Qunjie Xu, Honghua Ge and Junxi Zhang
Pages
691-700
DOI
10.4028/www.scientific.net/AMR.361-363.691
Citation
Y. Yang, S. X. Zhao, Y. Q. Xu, Z. Y. Yu, "Optimization and Comparison of Three Methods on Anthocyanins Extraction from Blackcurrant (Ribes nigrum L.) Using RSM", Advanced Materials Research, Vols. 361-363, pp. 691-700, 2012
Online since
October 2011
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