Paper Title:
Blue Fluorescent Quantum Dots-Based Probe for Detection of Influenza B Virus
  Abstract

Influenza is a common acute viral disease that has caused massive harm to human, so a rapid pathogen-detecting method is required for monitoring the status of the disease. Quantum dots are promising emerging fluorescent probe for diagnostic assay. In this work, water soluble ZnO quantum dots were covalently conjugated to rabbit anti-chicken IgY antibody to prepare the quantum dots-linked secondary antibody. The conjugate was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorescence spectrophotometer. Fluorescent quantum dots-based immunosorbent assay was conducted to determine the concentration of influenza B virus. We found that rabbit anti-chicken IgY antibody augmented its molecular weight while coupled to ZnO quantum dots, but the emission spectrum didn’t change. More importantly, the luminescence intensity of QDs correlated to viral concentration in a linear relationship. These results may suggest a potential new approach for quantifying virus, pathogenic bacteria and toxic molecule.

  Info
Periodical
Chapter
Chapter 1: Materials Engineering and Industry
Edited by
Barry Tan
Pages
18-22
DOI
10.4028/www.scientific.net/AMR.365.18
Citation
J. L. Wen, D. G. He, S. Q. Zhao, "Blue Fluorescent Quantum Dots-Based Probe for Detection of Influenza B Virus", Advanced Materials Research, Vol. 365, pp. 18-22, 2012
Online since
October 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Ya Min Shi, Guo Guang Rong, Dan Ni Wang, Shu Lin Zhang, Yong Xin Zhu
Chapter 9: Sensors and Measurement
Abstract:Though techniques in medicine develop in a very fast pace, tuberculosis still bothers researchers for its extensive existence. It is urgent...
555
Authors: Irtiqa Syed, Santa Chawla
Additional Papers
Abstract:A novel one pot synthesis approach in oleic acid medium was employed to obtain monophasic ZnSe quantum dots (QD) of average size 3.7nm. The...
73