Paper Title:
Preliminary Research on Differentially Expressed Proteins in Hippocampus of Rats Induced by Chronic Immobilization Stress and the Effect of Xiaoyaosan
  Abstract

To select differentially expressed proteins in hippocampus of rats with pattern of Liver Qi stagnation and Spleen deficiency (LQSSD) induced by chronic immobilization stress (CIS), by applying the technique of protein chip and surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). Methods: The rats with pattern of LQSSD according to traditional Chinese medicine (TCM) were replicated by the method of CIS. After twenty-one days’ stress, the protein chip weak cation CM10 and SELDI-TOF-MS were applied in order to do mass spectral analysis of the proteins acquired from the three groups: normal control group, 21 days model group, and Xiaoyaosan (XYS) treatment group. Bio Marker Wizard software were adopted to analyze the differentially expressed proteins. Results: Five differentially expressed proteins were selected between the control group and model group, and they were all highly expressed in the model group. Thirteen one were selected between control group and treatment group, among which 3 were highly expressed in the treatment group, and 10 were highly expressed in the control group. Only 1 differentially expressed protein was selected between the treatment group and the model group and it was highly expressed in the latter. Conclusion: The differentially expressed proteins may be the specific marker proteins in hippocampus of rats with pattern of LQSSD induced by CIS. TCM formula XYS may be an effective intervention for protein expression in hippocampus of rats with pattern of LQSSD.

  Info
Periodical
Chapter
Chapter 2: Future Materials Technology and Biomedicine
Edited by
Barry Tan
Pages
216-221
DOI
10.4028/www.scientific.net/AMR.365.216
Citation
M. J. Kou, D. C. He, P. Zhang, J. X. Chen, S. X. Wang, X. Zhao, M. H. Bai, X. L. Guo, "Preliminary Research on Differentially Expressed Proteins in Hippocampus of Rats Induced by Chronic Immobilization Stress and the Effect of Xiaoyaosan", Advanced Materials Research, Vol. 365, pp. 216-221, 2012
Online since
October 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Ana P. Felício, Eliandre de Oliveira, Maria A. Odean, Oswaldo Garcia Jr., Maria C. Bertolini, Lúcio F.C. Ferraz, Fernanda C. Reis, Laura M.M. Ottoboni, Maria T.M. Novo
Abstract:Acidithiobacillus ferrooxidans is used in bioleaching industrial operations to recover metal ions from mineral sulfides. Chalcopyrite and...
461
Authors: Ana P. Felício, Eliandre de Oliveira, M.A. Odena, Oswaldo Garcia Jr., Maria C. Bertolini, Lúcio F.C. Ferraz, Laura M.M. Ottoboni, Maria T.M. Novo
Abstract:The Acidithiobacillus ferrooxidans periplasmic space is known to have proteins involved in the respiratory chains. There are no reports about...
179
Authors: Fei Wang, Yu Geng, Wei Ming Zhang, Xin Geng
Session 2: Biomaterial Science
Abstract:To identify Zinc-alpha-2-glycoprotein (ZAG) expression in HCC for serum biomarker, by analyzing the serum proteome of the patients suffering...
383
Authors: Fei Wang, Yu Geng, Wei Ming Zhang, Xin Geng
Session 2: Biomaterial Science
Abstract:To Analyze the structure and function of Zinc-alpha-2-glycoprotein (ZAG), a highly expressed serum protein in the patients with...
390
Authors: Chi Chen, Yan Yan Liu, Zhi Ping Wu, Rui He Niu, Jian Zhong Tan
Chapter 1: Mulberry and Silkworm
Abstract:The silkworm fat body is the organ of many intermediary metabolic processes and sustenance of growth throughout the life, and its proteins...
67