Corn stalk was divided into fractions by morphological characters, and the enzymatic hydrolysis of these fractions was investigated. Chemical composition and the proportions of cells of different compositions were very variable in different morphological structure fractions. This has a fundamental effect on the enzyme adsorption capability and hydrolysis performance. Cellulase, β-glucanase and xylanase were added to the husks, leaves, pith and whole stalks of cornstalks. The results of enzymatic hydrolysis of different morphological structure fractions of corn stalk were compared. It can be seen that the content of sugar degraded by β-glucanase is the highest. The fastest hydrolytic rate and the highest sugar content were obtained in the pith of corn stalks.