A Pilot-scale study was conducted to treat municipal wastewater by Biological-Ecological filter (BEF), which is composed by anaerobic hydrolysis pool (AHP), high load biological filter (BF) and vermifilter (VF). The results demonstrated that when the influent concentrations of COD and NH3-N were in the range of 190~300 mg/L and 25~35 mg/L, respectively, the effluent concentration of them ranged from 45 to 65 mg/L and 8 to 20 mg/L with the average removal efficiency of 73.6% and 50%, respectively. Meanwhile, the effluent average SS concentration was less than 15 mg/L. The effluent quality could steadily meet the second level criteria specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB 18918-2002) in China. The removal performance of fine zeolite was significantly better than the coarse one and heightening packing layer had little effect on the removal of COD and NH3-N. This study verified that BEF system for municipal wastewater treatment was feasible.