Paper Title:
Preparation and Characterize of Konjac Glucomannan-Based Protein Molecularly Imprinted Polymer
  Abstract

Objective: To prepare a novel molecularly imprinted materials for protein recognition. Methods: Konjac glucomannan was used as fundamental materials, after swollen, added 3-Chloro-1,2- epoxypropane and glycerol cross-linked for 3 h at 40 °C. Then added bovine serum albumin (BSA) solution, agitated 1 h at room temperature. Natural dried. Results: After eluted, the imprinted efficiency of the imprinted membrane were (59.27±3.53) % (n=6) with blank membrane as control. The adsorption rate increased from 2 h to 20 h, and reached saturation at 22 to 24 h. The selectively recognition experiment indicated the BSA imprinted membrane have significant selectively recognition abilities for the template protein. Conclusions: konjac glucomannan-based imprinted membrane shows great possibilities to be a promising material for protein molecularly imprinted recognition.

  Info
Periodical
Chapter
Chapter 2: Material Science and Manufacturing Technology
Edited by
Helen Zhang and David Jin
Pages
460-463
DOI
10.4028/www.scientific.net/AMR.366.460
Citation
Z. Y. Mi, Z. Ma, X. L. Li, P. Wang, Y. Q. Zhang, "Preparation and Characterize of Konjac Glucomannan-Based Protein Molecularly Imprinted Polymer", Advanced Materials Research, Vol. 366, pp. 460-463, 2012
Online since
October 2011
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Price
$32.00
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