Paper Title:
Effect of Kanamycin Sulfate and Gentamicin on Growth of Probiotics
  Abstract

Effect of kanamycin sulfate and gentamicin on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus bulgaricus was studied by measuring the optical density at 600nm and pH using MRS broth as the control. The addition of kanamycin sulfate (%, w/v) was 0.0025, 0.005, 0.0075 and 0.01 and the addition of gentamicin(U/ml) was 48, 72, 144, 192 and 240. Results were as follows: addition of kanamycin sulfate at the concentration above 0.0025% has a significant inhibition on the growth of B. bifidum and has no influence on the other four probiotics at incubation12h or 24h. The optimum selective concentration of kanamycin sulfate in MRS media was 0.005% for selective enumeration of B.bifidum. Addition of gentamicin at the concentration above 240U/ml has a significant inhibition on the growth of L. bulgaricus at incubation 24h. Addition of neomycin sulfate in MRS can use to realize selective enumeration of L. bulgaricus in yogurt

  Info
Periodical
Chapter
Chapter 2: Material Science and Manufacturing Technology
Edited by
Helen Zhang and David Jin
Pages
490-493
DOI
10.4028/www.scientific.net/AMR.366.490
Citation
G. W. Shu, Z. Ji, Z. W. Wang, H. Chen, "Effect of Kanamycin Sulfate and Gentamicin on Growth of Probiotics", Advanced Materials Research, Vol. 366, pp. 490-493, 2012
Online since
October 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: He Chen, Li Yuan Ji, Guo Wei Shu, Zhao Wei Wang
Abstract:Effect of Lithium Chloride (LiCl) and Sodium Propionate (CH3CH2COONa) on growth of Lactobacillus acidophilus, Bifidobacterium bifidum,...
66
Authors: He Chen, Man Hu, Guo Wei Shu, Tao Qin, Jiang Ping Wang
Abstract:Effect of steroidal saponins fromFructus tribuli on growth of Bifidobacterium bifidum BB01, BB03 and Lactobacillus acidophilus LA09 was...
70
Authors: Guo Wei Shu, Zhen Xing Ma, Zhao Wei Wang, He Chen
Chapter 1: Material Engineering and its Application
Abstract:Effect of bile and nalidixic acid on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus...
248
Authors: Zong Cheng Miao, Yong Ming Zhang, Deng Wu Wang, Su De Ma, Zhong Jin Li
Chapter 2: Material Science and Manufacturing Technology
Abstract:Effect of steroidal saponins and flavonoid from Fructus tribuli on growth of Lactobacillus brevis was studied by measuring the optical...
506
Authors: He Chen, Li Yuan Ji, Guo Wei Shu, Chuan Na Li
Chapter 3: Energy Application, Material Engineering and Environment
Abstract:Effect of Galaeto-oligosaccharide (GOS) and Isomalto-oligosaccharide (IMO) on growth of Lactobacillus acidophilus, Bifidobacterium longum and...
450